Activated Oxygen Sensitive Hydrogel
A double selenium bond-bridged burdock sapogenin (ATG)-chitosan (CS) copolymer and further made into a ROS-sensitive hydrogel (ATG-CS-Gel).
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Experimental parameters
| Dissolution apparatus | RT612-ST Automated Dissolution System |
| Method | A dissolution cup containing 200 mL of PBS (pH 7.4, 2% SDS) with or without (0.4% H₂O₂) |
Experimental results
ATG-CSATG-CS (11 % modification) was selected as the optimal prescription because of the breakage of the diselenide bond in 0.4 % H2O2 solution, the fastest ROS-responsive drug release within 1 h (81.6 % in 4 h), and the highest water solubility and swelling rate.
In subsequent pharmacodynamic evaluations, the prescription demonstrated excellent therapeutic effects in both Caco-2 cells and a mouse model of colitis: e.g., adherence to the colonic lesion for up to 24 hours; superiority over the control drug mesalazine in improving the activity index, colon length, and intestinal tissue damage in mice; and a high potential for repairing the intestinal mucosal barrier by modulation of MLCK, among others. These results indicate that the results of in vitro drug release, water solubility, and dissolution rate tests do provide strong support for subsequent biologically relevant tests.
CONCLUSION: Raytor RT612-ST Automated Dissolution System, on the other hand, accurately predicts the optimal prescription, improves experimental efficiency and reduces the number of experimental animals.
